711 / 2019-05-06 12:23:43

The molecular mechanism of AtPRMT3 in promoting proper pre-rRNA processing

AtPRMT3, RPS2, pre-rRNA processing

全体主题 > RNA Biology

Ribosome biogenesis is a fundamental process in all cells and essential for growth and development in metazoans. It includes rDNA transcription, pre-rRNA processing, modification as well as assembly with ribosomal proteins and associated factors. The regulatory mechanisms of ribosome biogenesis have been well deciphered in yeast, however much are largely unknown in higher plants.
AtPRMT3, which is a member of protein arginine methyltransferases, functions in ribosome biogenesis by regulating proper pre-rRNA processing, however the molecular mechanism remains unknown. Here we identified that RPS2 can interact with AtPRMT3. RPS2 protein family consists of four members, including RPS2A, RPS2B, RPS2C and RPS2D, these four members are highly conserved. We found that rps2 mutant showed similar developmental defects and aberrant pre-rRNA processing to atprmt3. Our findings revealed the molecular mechanism by which AtPRMT3 and RPS2B regulate pre-rRNA processing during ribosome biogenesis, shedding light on the mechanisms of PRMT3 and RPS2 function in ribosome biogenesis for other species.